Optimizing a biomass-based hydrogen bioproduction process offers a promising alternative to hydrogen production using energy-intensive thermochemical processes based on fossil fuels. In this study, biomasses with a high annual production tonnage in France (beet pulp, waste fruit and vegetables, grass silage, methanizer input mix), generally valorized by anaerobic digestion for methane production, were selected as test inputs for hydrogen bioproduction by dark fermentation. The endogenous production method, i.e. with no added inoculum and no pre-treatment, was tested in a batch reactor (Renaudie et al.). This initial screening highlighted two particularly interesting biomasses: beet pulp, with the highest H2 yield (31 L H2/kg biomass), and fruit and vegetable juice, for its H2 yield (15.7 L H2/kg biomass) and high productivity (75 mL H2/L/h). Grass silage remains a remarkable biomass for its significant yield and productivity, given the large tonnage available in the region. Receiving input for methanization gives significantly lower production results in dark fermentation. For this biomass, the use of more than 40% manure in this mixture limits the carbohydrate content and potentially introduces microorganisms that are not favorable to H2 production; it should be remembered that no heat treatment is carried out prior to fermentation. A study of the impact of various parameters controlled during this process (freezing, biomass content in the reaction medium, pH regulation, base addition, medium agitation, sweep gas and bacterial enrichment) on beet pulp biomass revealed that pH regulation is the most critical parameter, followed by the use of a sweep gas such as carbon dioxide. The metabolisms of substrate consumption and production of various metabolites were studied, as well as the impact on the composition of the microbial consortium. The distribution of biomass bacterial diversity changed radically between the initial moment and after dark fermentation, with the emergence of bacteria from genera (Clostridium, Enterobacter) referenced as hydrogen producers, constituting the originality of this study.